1Novia Aprilia Rahma, 2Hadi Sarosa, 3Joko Wahyu Wibowo
1,2,3Faculty of Medicine, Sultan Agung Islamic University, St Kaligawe KM 4 Semarang 50012, Indonesia
DOI : https://doi.org/10.47191/ijmra/v6-i12-75Google Scholar Download Pdf
ABSTRACT:
Hypercholesterolemia is a condition by an increase total cholesterol and Low Density Lipoprotein (LDL) cholesterol levels, which can lead the secretion of proinflammatory cytokines Interleukin-6 (IL-6) through the activation of the NF-kB pathway oxidative stress, and increase in Malondialdehyde (MDA) levels. Studies showed that the extract of bitter melon contains flavonoids as antioxidants can reduce cholesterol levels. To find out the effect of bitter melon extract on total cholesterol levels, IL-6, and MDA in hypercholesterolemic rats. Experimental research with Post Test Only Group Design. The research subjects were 25 wistar rats divided into 5 groups. Group K(N) normal rats, K(-) were only given a hypercholesterol diet for 28 days, groups K(+), P1, and P2 were given a hypercholesterol diet for 14 days followed by 14 days of hypercholesterol diet along with simvastatin at 0.09 mg/kgBW, bitter melon extract at 150 mg/kgBW, and combination of bitter melon extract at 75 mg/kgBW and simvastatin at 0.045 mg/kgBW. On the 29th day the rats were examined. The results of the Shapiro-Wilk and Levene tests showed that the data were normally distributed and homogenous. One-Way ANOVA and Tukey test was found that the levels of cholesterol, IL-6, and MDA in the P1, P2, and P3 hypercholesterolemic rat groups underwent significantly (p0.05) compared to the control group. The extract of bitter melon, simvastatin, and the combination of simvastatin and bitter melon extract have effect on reducing cholesterol levels, IL-6, and MDA in hypercholesterolemic rats.
KEYWORDS:Bitter Melon, Interleukin-6, Malondialdehyde, Total Cholesterol, Simvastatin
REFERENCES1) Andiani and Harsa, M. S. (2018) ‘Effect administration of ethanol extract of bitter melon (momordica charantia l.) on the reduction of MDA (malondialdehyde) levels serum in rats given a high-fat diet’, Jurnal Ilmiah Kedokteran Wijaya Kusuma, 7(2), p. 196. doi: 10.30742/jikw.v7i2.466.
2) Aprilia, C. A. and Marlina Dewiastuti (2018) ‘Efektivitas Hipolipidemia dan Antioksidan Ekstrak Daun Binahong pada Tikus Putih yang Diinduksi Pakan Hiperkolesterol’, YARSI Medical Journal, 25(3), p. 150. doi: 10.33476/jky.v25i3.362.
3) Artha, C., Mustika, A. and Sulistyawati, S. W. (2017) ‘Pengaruh ekstrak daun singawalang terhadap kadar ldl tikus putih jantan hiperkolesterolemia’, eJournal Kedokteran Indonesia, 5(2), pp. 105–109. doi: 10.23886/ejki.5.7151.
4) Binmowyna, M. N. et al. (2021) ‘Hypolipidemic and antioxidant effects of the juice and water seed extracts of two pomegranate species in high-cholesterol diet fed rats’, Food Science and Technology, 41(December), pp. 732–740. doi: 10.1590/fst.31220.
5) Chaturvedi et al. (2004) ‘Effect of momordica caharantia on lipid profile and oral glucose tolerance in diabetic rats’, Phytother Res, 18(11), pp. 956–6.
6) Darwin, E., Afriani, N. and Hanam, N. N. (2016) ‘Pengaruh arginin terhadap kadar IL_6, IL-17, dan TGF-B pada rattus novergicus dengan diet tinggi lemak’, PIN PAAI, pp. 130–136.
7) Destiana, D. and Timan, I. S. (2018) ‘The relationship between hypercholesterolemia as a risk factor for stroke and blood viscosity measured using digital microcapillary’, Journal of Physics: Conference Series, 1073(4). doi: 10.1088/1742-6596/1073/4/042045.
8) Dixon et al. (1998) ‘The effect of low carotenoid diet on malondialdehyde-thiobarbituric acid (MDA-TBA) concentrations in women: a placebo-controled double blind study’, J. AM. Coll. Nut, 62, pp. 149–150.
9) Fernández-Real, J. M. et al. (2000) ‘Interleukin-6 gene polymorphism and lipid abnormalities in healthy subjects’, Journal of Clinical Endocrinology and Metabolism, 85(3), pp. 1334–1339. doi: 10.1210/jc.85.3.1334.
10) Jia, S. et al. (2017) ‘Recent advances in momordica charantia: Functional components and biological activities’, International Journal of Molecular Sciences, 18(12). doi: 10.3390/ijms18122555.
11) Kumar, S., Singh, U. N. and Dhakal, S. (2017) ‘Study of oxidative stress in hypercholesterolemia’, International Journal of Contemporary Medical Research, 4(5), pp. 2454–7379. Available at: www.ijcmr.com.
12) Kurniawaty and Liani, S. (2013) ‘Pengaruh pemberian ekstrak biji jengkol (pithecellobium lobatum benth.) terhadap kadar kolesterol total dalam darah tikus diabetes yang di induksi aloksan’, Medical Faculty of Lampung university, 4, pp. 70–76.
13) Kusuma, A. M. et al. (2017) ‘Efek ekstrak bawang dayak (eleutherine palmifolia (l.)merr) dan ubi ungu (ipomoea batatas l) terhadap penurunan kadar kolesterol dan trigliserida darah pada tikus jantan’, Jurnal Kefarmasian Indonesia, 6(2), pp. 108–116. doi: 10.22435/jki.v6i2.6225.108-116.
14) Maulida, M. (2021) Pengaruh pemberian VCO ( virgin coconut oil ) terhadap kadar kolesterol HDL ,LDL, dan IL-6, Universitas Islam Sultan Agung Semarang.
15) Purnamasari, A. W. and Isnawati, M. (2014) ‘Pengaruh pemberian jus pare (momordica charantia l.) dan jus jeruk nipis (citrus aurantifolia) terhadap kadar kolesterol total tikus sprague dawley hiperkolesterolemia’, Journal of Nutrition College, 3(4), pp. 647–654.
16) Purwantyastuti (2000) Relation of lipid peroxide to food habits, selected coronary heart disease risk factors and vitamin e suplementation in the elderly. UI.
17) Rahma, N. L. and Syauqy, A. (2013) ‘Pengaruh pemberian jus biji pepaya (carica papaya linn.) terhadap kadar trigliserida tikus sprague dawly dislipidemia’, Journal of Nutrition College, 2(3), pp. 118–125. Available at: http://ejournal-s1.undip.ac.id/index.php/jnc.
18) Rahmawati, Y. et al. (2022) ‘Hiperkolesterolemia pada pasien lanjut usia’, Jurnal Kesehatan Tambusai, 3, pp. 157–163.
19) Saraswati, R. D. and Khariri (2021) ‘Transisi epidemiologi stroke sebagai penyebab kematian pada semua kelompok usia di indonesia’, Seminar Nasional Riset Kedokteran, 2(1), pp. 81–6. Available at: https://conference.upnvj.ac.id/index.php/sensorik/article/view/1001.
20) Sarihati, I. G. A. D. and Dhyanaputri, I. G. A. S. (2020) ‘Pengaruh Ekstrak Etanol Daun Kenikir (Cosmos caudatus) terhadap Kadar Glutathion Dan Interleukin 6 Serum Tikus Wistar Jantan yang Diberi Pakan Tinggi Kolesterol’, Jurnal Kesehatan, 11(1), p. 77. doi: 10.26630/jk.v11i1.1836.
21) Simorangkir, D. and Wahyudi (2021) ‘Formulasi dan uji efektivitas antihiperlipidemia kombinasi ekstrak etanol buah pare (momordica charantia l.) dan jahe merah (zingiber officinale rosc var.rubrum) terhadap tikus jantan putih (rattus norvegicus)’, Jurnal Biosains (The Journal of Biosciences), 7(3), pp. 115–120. Available at: https://doi.org/10.24114/jbio.v5i2.13984%0AISSN.
22) Tubagus, T. A., Momuat, L. I. and Pontoh, J. S. (2015) ‘Kadar kolesterol plasma tikus wistar pada pemberiak ekstrak etanol dan heksana dari daun gedi merah ( abelmoschus manihot)’, Jurnal MIPA UNSRAT, 4(1), pp. 63–68.
23) Vijayan, J. et al. (2018) ‘Hypercholesterolemia’, European Journal of Biomedical and Pharmaceutical Sciences, 5(3), pp. 245–249.
24) Wahjuni, S. (2015) Dislipidemia menyebabkan stress oksidatif ditandai oleh meningkatnya malondialdehid, Udayana Unerversity Press. Edited by I. H. Utama. Denpasar, Bali.
25) Wulandari, D. Y., Padaga, M. C. and Herawati (2012) ‘Kadar MDA dan gambaran histopatologi organ hati tikus hiperkolesterolemia setelah terapi ekstrak air benalu mangga’, Jurnal Medik Veteriner Universitas Brawijaya. Available at: https://fkh.ub.ac.id/wp-content/uploads/2012/10/0911310007-DebinYuniarW.pdf.
26) Yu, G. J. et al. (2015) ‘Anti-inflammatory potential of saponins derived from cultured wild ginseng roots in lipopolysaccharide-stimulated RAW 264.7 macrophages’, International Journal of Molecular Medicine, 35(6), pp. 1690–8. doi: 10.3892/ijmm.2015.2165.
27) Zeng, Y. et al. (2018) ‘Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation’, Lipids in Health and Disease. Lipids in Health and Disease, 17(1), pp. 1–7. doi: 10.1186/s12944-018-0896-0.
Volume 06 Issue 12 December 2023
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